Fig. 3. Effects of GCDC on Src kinases in the perfused rat liver. Livers were perfused with Krebs-Henseleit buffer with or without GCDC (20 µmol/l) in absence or presence of apocynin (20 µmol/l), SU6656 (1 µmol/l), NAC (10 mmol/l) or PP-2 (250 nmol/l) as indicated in the perfusion plan. At the end of the experiment liver tissue was snap frozen, protein was isolated and used for Western blot analysis of activated Src kinase family members Yes, Fyn, and c-Src. Expression levels were quantified by densitometric analysis. Expression levels in livers perfused for 60 min with GCDC are given relative to what was found prior to perfusion with GCDC (0 min, "Control"). Representative blots and densitometric analysis of at least 3 independent perfusion experiments are shown. * statistically significantly different compared to control. n.s.: not statistically significantly different.